酪朊酸钠酶解液的分级膜分离的研究

Fractional Ultrafiltration Separation of Sodium Caseinate Peptides

利用截留不同分子质量(10ku、5ku、3ku和1ku)的超滤膜将水解度为11 5 %的酪朊酸钠水解液按分子质量大小分为5级,利用SDS -PAGE研究了酪朊酸钠水解液的分子质量分布及分级膜的分离效果。结果表明,分级膜分离方法对酪朊酸钠水解液中的混和肽类能按照分子量大小进行有效的分离。

The fractionation of sodium caseinate peptide by ultrafiltration separation was studied. Sodium caseinate peptide products (hydrolysis degree of 11.5%) can be separated into five components (>10 ku, 10～5 ku, 5～3 ku, 3～1 ku,<1 ku) in terms of molecular weight. The molecular weight distribution of each retentate was determined by SDS-PAGE. The result showed that fractional ultrafiltration separation can effectively separate sodium caseinate peptides.