纸层析-分光光度法测定发酵液中L-丝氨酸和甘氨酸的含量

Determination of L-Serine and Glycine in Broth with Paper Chromatography and Spectrophotometer

应用“纸层析 分光光度法”对发酵液中的L 丝氨酸和甘氨酸进行了定性分析和定量测定,确立了L 丝氨酸和甘氨酸定量测定条件。研究结果表明,该法对测定L 丝氨酸和甘氨酸含量具有较高的准确度和精密度。而且该法操作简单、易于掌握,非常适合在L 丝氨酸产生菌筛选中应用。

The concentrations of L-serine and glycine in broth were qualitatively and quantitatively determined with paper chromatography and spectrophotometer. The determination conditions of L-serine and glycine were established. It showed that the method is highly accurate and precise in the determination of L-serine and glycine. The method was easy to operate and suitable for the screening of strains producing L-serine.