低密度脂蛋白免疫复合物对巨噬细胞细胞因子分泌的影响

The effect of LDL-containing immune complexes on the secretion of cytokines in macrophages

目的探讨低密度脂蛋白免疫复合物(LDLIC)对巨噬细胞细胞因子分泌的影响。方法设立终浓度均为150μgml的LDL组、LDL-IC组、IgG-IC组及阴性对照组与细胞孵育相同时间,以ELISA法检测比较培养上清TNFα和IL1β水平。并观察不同LDLIC浓度(0,50,100,150,200,250μgml)作用相同时间以及终浓度为150μgml的LDLIC与细胞作用不同的时间(0,3,6,12,24,36h),培养上清TNF-α和IL-1β水平。结果LDL-IC组培养上清TNF-α、IL-1β水平均明显高于LDL、IgG-IC及阴性对照组。TNF-α、IL-1β水平随着LDL-IC作用浓度的增加和作用时间的延长而增高。结论LDL-IC可以显著增加细胞TNF-α和IL-1β的分泌,具有剂量和时间依赖效应。提示LDL-IC能通过增加细胞因子分泌而在动脉粥样硬化中起重要作用。

Objective To study the effect of LDL-containing immune complexes (LDL-IC) on the secretion of cytokines in macrophages. Methods Four matched groups were set namely 150 μg/ml of LDL-IC, native LDL, immune complexes of IgG(IgG-IC) and negative control, and incubated with macrophages for the same duration. The concentrations of TNF-α and IL-1β in the supernatant were determined by enzyme linked immunosorbent assay. Moreover, for investigating the changes of the secretion of the cytokines, the macrophages were incubated with different concentrations of LDL-IC ( 0,50,100,150,200,250 μg/ml) or with 150 μg/ml LDL-IC for different periods (0,3,6,12,24,36 h). Results The TNF-α and IL-1β levels of the cells treated with LDL-IC were higher than the groups treated with LDL?IgG-IC and negative control ( P <0.01). The TNF-α and IL-1β levels were increased with increasing LDL-IC concentration and prolonging incubation time. Conclusions The secretion of TNF-α and IL-1β was markedly increased after the cells were incubated with LDL-IC and there were dose-and time-dependent effects. LDL-IC may play an important role in the development of atherosclerosis by interference of cytokine secretion.