摘要
用生物降温仪、低温显微镜和细胞化学、细胞培养等技术研究证明,低温保存液的相变温度随降温速率的加快而增高,以1℃/min降温时,其相交点在—13.9℃,骨髓细胞浓度对相变温度无影响。液氮(—196℃)冻存后,外用血淋巴细胞体积无明显变化,其糖原和非特异性酯酶含量明显降低。胎肝细胞冻存1098天染色体正常,冻存930天后氧耗量与新鲜值无明显差别,冻存7天后超微弱发光强度为新鲜值的92％。小鼠骨髓和皮肤冻存后,移植免疫排斥反应降低。人骨髓冻存3～300天,胎肝冻存1～1257天,外周血冻存6～53天,其CFU—GM回收率分别为68.9％、62.0％和77.3％。造血细胞活力不随保存时间延长而降低。
; Cryobiological effects of bone marrow, fetal liver and peripheral blood hemopoi-etic stem cells were studied by means of biological freezer, cryomicroscope, as well as cyto-chemical and cell culture techniques. The phase change temperature of cryoprotective solution rises with the increase of vooling rate. When freezing rate is 1℃ /min, phase change temperature is -13. 9 ℃, After cryopreservarion the diameters of peripheral blood lymphocytes have not change apparently, wherase the contents of glycogen and nonspecific esterase positive rate have been decreased obviously. The chromosomes, oxygen consumption and ultra-weak luminosity of fetal liver cells had remained normal for 1098, 930 and 7 days' cryopre-served respectively. Less HvGR of murine bone marrow and skin occured, and more CFC-GM of human bone marrow fetal liver and peripheral blood were harvested after cryopreser-vation. The viability of hemopoietic cells doesn' t decrease with the time of cryopreservation.
出处
《制冷》
1994年第2期54-58,共5页
Refrigeration
