胃癌中E-cadherin基因甲基化状况及其表达的研究

METHYLATION OF GENE E-CADHERIN AND ITS EXPRESSION IN GASTRIC CARCINOMAS

目的:检测胃癌组织中E-cadherin基因启动子区域的甲基化状况,并探讨基因异常甲基化与蛋白表达及其临床病理指标的关系。方法:采用甲基化特异性PCR方法对胃黏膜中E-cadherin基因启动子区域甲基化状况进行检测,并采用免疫组织化学方法相应检测了E-cadherin蛋白表达。结果:胃癌中E-cadherin基因甲基化率为3 4.6% (2 8/81 ) ,显著高于正常胃黏膜该基因甲基化率(P =0 .0 2 8)。E-cadherin蛋白表达阳性率为64.2 % (5 2 /81 )。E-cadherin基因甲基化与其蛋白表达无相关性(P >0 .0 5 ) ,与肿瘤组织学类型、淋巴结转移及性别均无相关性,但与年龄有关,随着年龄增加病人甲基化率呈增高趋势。结论:E-cadherin基因甲基化与年龄有关,E-cadherin基因异常甲基化是常见的一种基因异常改变,可能参与了胃癌的发生发展过程。

Objective: To investigate the promoter methylation status of gene E-cadherin, its expression at protein level in gastric carcinomas, and its correlation with clinicpathological parameters. Methods: The promoter methylation of E-cadherin of a series of 81 gastric carcinamas and 10 normal gastric tissues was examined by means of methylation-specific PCR. The expression of E-cadherin was detected by immunohistochemistry. Results: Methylation of E-cadherin was deterted in 34.6%(28/81) of gastric carcinomas, which was significantly higher than the normal methylation of E-cadherin ($P$=0.28), and the positive immunostaining for E-cadherin protein was found in 64.2% (52/81) of gastric carcinomas. a higher methylation of E-cadherin was detected with the in increase in age. But it was not associated with different status, sex and lymph node metastasis. Conclusion: Methylation of E-cadherin is associated with age, and it is a common event in gastric carcinomas and may play a role in the development of gastric carcinomas.;