摘要
以闽楠叶片为材料,研究了闽楠基因组DNA提取方法及RAPD反应条件。采用改良的CTAB高盐法提的DNA质量较高,适宜RAPD-PCR分析。在20μL反应体积中,RAPD分析的优化反应体系为:2mmol/L的Mg2+,200μmol/LdNTP,1.5ng/μL的模板DNA,1UTap酶,50nmol/L引物。
The The methods of genomic DNA extraction and the optimizing conditions of RAPD analysis in Phoebe bournei were tested.The results indicated that by using the method of CTAB with high salt preipitation the high grade of genomic DNA was obtained;and the optimal PCR system for RAPD analysis was as follows:2mmol/LMg2+ ,200umol/L dNTP,1.5ng/μL DNA template,1U Tap polymerase,50nmol/L random primer in 20μL reaction solution.
出处
《江西林业科技》
2005年第2期5-7,共3页
Jiangxi Forestry Science and Technology
基金
江西省自然科学基金项目"闽楠群体遗传多样性研究(0030030)"
宁波青年基金项目"观赏植物新品种分子标记选育与造型栽培研究(OJ20300-28)"内容
关键词
闽楠
DNA提取
RAPD
优化
Phoebe bournei
DNA extraction
RAPD
optimization.
