克拉霉素与醌茜素的荷移反应及其测定

The Spectrophotometric Determination of Clarithromycin Based on the Charge Transfer Reaction between Clarithromycin and Quinalizarin

克拉霉素与醌茜素在乙醇 -水介质中发生电荷转移反应 ,形成 1∶ 1的电荷转移络合物。该络合物在5 80 nm处有最大吸收 ,表观摩尔吸光系数为 3.74× 1 0 3 L·mol-1· cm-1 ,稳定常数是 2 .6× 1 0 5。建立了一种快速测定克拉霉素的荷移分光光度法。药物浓度在 0— 1 0 0 mg/ L范围内服从比耳定律。本法测定了克拉霉素制剂中有效成分的含量 ,与文献法结果基本吻合。

A rapid spectrophotometric method for the determination of clarithromycin was developed based on the 1∶1 charge transfer complex between clarithromycin as donor and quinalizarin as acceptor in water-alcohol solution with stability constant of 2.6×105 and apparent molar absorptivity of 3.74×103L·mol -1·cm -1 at 580nm.Beer's law is obeyed in the range of 0—100mg/L of clarithromycin with correlation coefficient is 0.9995.The average standard deviation of six determinations is 1.34% at the concentration of clarithromycin of 50mg/L.The recovery is over 98%.The method was applied to the determination of clarithromycin in tablets with satisfactory results.