广东2003年末SARS患者冠状病毒抗原抗体动态特征分析

Dynamic observation to SARS-CoV antibody and antigen in late 2003 in Guangdong

目的 分析SARS IgG、SARS IgM、核壳蛋白 IgG抗体和核壳蛋白抗原的变化特点并探讨其意义。方法 采用酶联免疫方法对 2 0 0 3年末至 2 0 0 4年初新发 4例SARS病人的系列血清进行上述 4项的滴度检测。结果 在病人较早期的血清中可以检测到核壳蛋白抗原 ,随着抗体水平的升高 ,抗原含量迅速下降。抗体上升下降快 ,在最高水平维持时间短 ,并且除第 1例外 ,其余 3例的抗体滴度均小于 1∶1 0 0 ,核壳蛋白抗体变化规律与总的IgG抗体一致 ,但滴度更低。结论 抗体变化规律与前次流行不同 ,抗体水平变化快 ,应注意及时采集标本。抗原检测可作为一种实验室诊断依据。核壳蛋白抗体可考虑用于早期诊断。

Objective To analyze kinematics of SARS-IgG, SARS-IgM, nucleocapsid protein and its IgG in new cases meanwhile to explore their significance. Method Serial sera from 4 patients were collected. The titer of the four items were detected by ELISA. Results Nucleocapsid protein in sera can be detected in early stage, then decreased quickly, while antibody increasesd quickly. But the antibody began to decrease in a short time after reaching the peak, and the titer was lower than 1∶100 in 3 cases. The antibody to nucleocapsid protein changes in accordance with SARS-IgG with lower titer. Conclusion The kinematics of SARS antibody was different with that in last outbreak. Antigen test can be used for laboratory diagnosis of SARS. Antibody to nucleocapsid protein may be a potential target for early diagnosis.