环境致癌加合物MNU－DNA的高效液相色谱分离与质谱鉴别

Mass Spectrometric Identification and HPLC Separation of Environmental Carcinogen MNU-DNA Adducts

本文报道环境致癌物ＭＮＵ与ＤＮＡ形成的ＭＮＵ－ＤＮＡ加合物的ＨＰＬＣ与ＭＳ的分离、鉴别结果。加合物在水解后分析。ＨＰＬＣ法通过保留时间及各洗提物的ＵＶ吸收光谱在线检测，筛选出感兴趣的目标化合物（Ｎ７－ＭＧ，Ｏ６－ＭＧ），再通过质谱（ＥＩＭＳ及ＦＡＢＭＳ）鉴定。

The experimental results of analysis of adducts formed from environmental carcinogenic compound MNU-DNA by combination of HPLC and FABMS are presented in this paper. The analysis of the adducts is carried out after hydrolysis of MNU - DNA adduct. The HPLC efflunts are screened with their UV spectra by DAD and the efflunts of intereste O6 - MG and N7 - MG are collected for MS analysis. The hydrolytical efficiency of DNA and MS fragmental paths of DNA bases adducts are also discussed.