髓腔内恒定低压直流电刺激修复家兔桡骨缺损的实验研究

Study on repairing of the rabbit radial defect with direct galvanic stimulation in medullary cavity

目的 探讨髄腔内恒定低压直流电刺激对家兔桡骨缺损的修复作用及其临床应用价值。方法 将去抗原牛松质骨(DBX)植入家兔桡骨节段性骨/骨膜缺损模型中,随机分为两组:A组(实验组):给予骨缺损区髄腔内恒定低压直流电持续刺激;B组(对照组):不作任何处理。分别于2、5、9周取材,测定血清钙离子浓度和碱性磷酸酶(ALP)含量,行X线拍片、组织形态学及电镜观察。结果 实验组同术前和对照组相比:血清钙离子浓度及ALP含量升高,有显著性差异(P<0.01);成骨细胞及微血管增生活跃; 9 周新生骨桥接缺损,有骨改建进行;骨超微结构基本正常。结论 髄腔内恒定低压直流电刺激能明显促进骨缺损愈合与修复,有良好的临床应用前景。

Objective To explore the effect of low constant direct current (LCDC) in the medullary cavity on repairing of rabbit radial defect as to evaluate its clinical value. Methods DBX was put into the segmental osteoperiosteum defect of rabbit bilateral radius. The rabbits were divided into 2 groups: group A (subject group) with the defects stimulated by LCDC and group B (control group) with the defects left untreated. In week 2,5 and 9, 4 animals from each group were put into death, and the radius was taken for study using photograph, electron microscope, histological & morphologic technique, and the contents of the Calcium (Ca) and ALP were determined. Results Compared with group B, the healing of group A was better. The contents of Ca and ALP increased obviously in week 2 (P< 0.01 ), and osteoblasts and growth of capillary showed higher vitality. In the meantime, bone bridging and bone remodeling took place, and bone ultrastructures were normal. Conclusion LCDC is effective in repairing the bone defects and it has a good clinical value.