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牛血清白蛋白对小鼠原核期胚胎玻璃化冷冻的影响 被引量：2

Effect of Bovine Serum Albumin on Vitrification of Pronuclear-Stage Mouse Embryo
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摘要 以小鼠原核期胚胎为对象,以胚胎的存活率、卵裂率、囊胚率以及囊胚细胞数作为检测指标,在M2液的基础上添加8种浓度(0,2,4,8,16,32,64,96mg/mL)牛血清白蛋白(BSA)配置防冻液,探讨防冻液和玻璃化冷冻后对胚胎发育的影响。BSA防冻液对胚胎发育影响的实验结果表明,8个浓度组间以及与对照组间胚胎的卵裂率、囊胚率以及囊胚细胞数无显著差异(P>0.05),说明在防冻液中加入一定浓度的BSA对小鼠胚胎无毒性作用。防冻液经玻璃化冷冻后对胚胎发育影响的实验表明,8个浓度组间冷冻胚胎复苏后的存活率、卵裂率、囊胚率及囊胚细胞数无显著差异(P>0.05)。表明BSA在这种防冻液中没有明显的保护作用。从经济、实用、生物安全角度考虑,不支持在玻璃化防冻液中添加BSA。 The cryoprotective effect and toxicity of Bovine Serum Albumin (BSA) at 8 concentrations (0, 2, 4, 8, 16, 32, 64, 96 mg/mL, respectively) supplemented in cryoprotectant solutions on mouse pronuclear-stage embryo vitrification were studied. Survival rate, cleavage rate, blastocyst rate and mean cell numbers of blastocyst of vitrified embryos were used as criterion to evaluate the effect of BSA on the post-thawed embryos development. The results showed that no statistical differences of survival rate, cleavage rate, blastocyst rate and mean cell numbers of blastocyst of vitrified groups both in the toxic effect assay (P>0.05) and the vitrification assay (P>0.05). The results indicated that BSA in this cryoprotectant solutions has no effect on the viability of pronuclear-stage embryos. Economical, practical and biosecure considerations do not support the use of BSA in vitrificationsolutions.
作者 关沫 杨世华 司维 采克俊 季维智
机构地区 中国科学院昆明动物研究所
出处 《Zoological Research》 CAS CSCD 北大核心 2005年第2期197-202,共6页 动物学研究（英文）
基金 国家自然科学基金资助项目(30370166) 国家重点基础研究发展规划项目(G2000016108) 中国科学院知识创新工程资助项目(KSCX1-05)
关键词 牛血清白蛋白 玻璃化冷冻 原核期胚胎 小鼠 存活率 卵裂率 囊胚率 囊胚细胞数 BSA Vitrification Pronuclear-stage embryos Mouse
分类号 S865.13 [农业科学—野生动物驯养]
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参考文献25

  • 1Alvarez JG, Storey BT. 1993. Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: Glycerol and other polyols as sole cryoprotectant[J]. J. Androl., 14(3): 199-209.
  • 2Bagis H, Sagirkaya H, Odaman MH, Dinnyes A. 2004. Vitrification of pronuclear-stage mouse embryos on solid surface (SSV) versus in cryotube: Comparison of the effect of equilibration time and different sugars in the vitrification solution[J]. Melecular Reproduction and Development, 67(2): 186-192.
  • 3Begin I, Bhatia B, Baldassarre H, Dinnyes A, Keefer CL. 2003. Cryopreservation of goat oocytes and in vivo derived 2- to 4-cell embryos using the cryoloop (CLV) and solid-surface vitrification (SSV) methods[J]. Theriogenology, 59(8): 1839-1850.
  • 4Bernart W, Kamel M, Neulen J, Breckwoldt M. 1994. Influence of the developmental stage and the equilibration time on the outcome of ultrarapid cryopreservation of mouse embryos[J]. Hum. Reprod., 9(1): 100-102.
  • 5Braun J, Hochi S, Oguri N, Sato K, Torres-Boggino F. 1995. Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa[J]. Cryobiology, 32(5): 487-492.
  • 6Cabrita E, Anel L, Herraez MP. 2001. Effect of external cryoprotectants as membrane stabilizers on cryopreserved rainbow trout sperm[J]. Theriogenology, 56(4): 623-635.
  • 7De Leeuw FE, De Leeuw AM, Den Daas JH, Colenbrander B, Verkleij AJ. 1993. Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing[J]. Cryobiology, 30(1): 32-44.
  • 8Dinnyes A, Dai YP, Jiang S, Yang XZ. 2000. High developmental rates of vitrified bovine oocytes following parthenogenetic activation, in vitro fertilization, and somatic cell nuclear transfer[J]. Biol. Reprod., 63(2): 513-518.
  • 9Erbach GT, Papaioannou VE. 1994. Differential growth of the mouse preimplantation embryo in chemically defined media[J]. Biol. Reprod., 50(5): 1027-1033.
  • 10Kasai M, Hamaguchi Y, Zhu SE, Miyake T, Sakurai T, Machida T. 1992. High survival of rabbit morula after vitrification in an ethylene glycol-based solution by simple method[J]. Biol. Reprod., 46(6): 1042-1046.

二级参考文献16

  • 1Martino A, Songsasen N, Leibo S P, et al. Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling[J]. Biol. Reprod, 1996, 54: 1059.
  • 2Maria Teresa Zenzes, Ryszard Bieleeki, Casper R F, et al,Effects of chilling to 0℃ on the morphology of meiotic spindies in human metaphase Ⅱoocytes[J]. Fertil and Steril,2001, 75: 769.
  • 3Palasz A T, Mapletoft R J. Cryopreservation of mammalian embryos and oocytes: recent advances[J]. Biotechnol. Adv.1996, 14: 127.
  • 4Vajta G, Booth P J, Holm P, Greve T, et al. Successful vitrification of early stage bovine in vitro produced embryos with the Open Pulled Straw(OPS) method[J]. Cryo-lett. 1997,18: 191.
  • 5Park S E, Chung H M, Cha K Y, et al. Cryopreservation of ICR mouse oocytes: improved post-thawed preimplantation development after vitrification using TaxolTM, a cytoskeletion stabilizer[J]. Fertil and Steril, 2001, 75: 1177.
  • 6Dobrinsky J R, Pursel V G, Long C R, et al. Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification[J]. Bio Reprod, 2000, 62: 564.
  • 7Hong S W, Chung H M, Lira J M, et al. Improved human oocyte development after vitrification: a comparison of thawing methods[J]. Fertil and Steril, 1999, 72: 142.
  • 8Mukaida T, Wada S, Takahashi K, et al. Vitrification of human embryos based on the assessment of suitable conditions for8-cell mouse embryos[J]. Hum Reprod, 1998, 13: 2874.
  • 9Michelle Lane, Schoolcraft, W B, Gardner, D.K., et al.Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique[J]. Fertil and Steril, 1999, 72:1073.
  • 10Vanderzwalmen, P, Bertin G, Debauche C H, et al. Births after vitrification at morula and blastocyst stages:effect of artificial reduction of the blastoeoelie cavity before vitrification[J].Hum Reprod, 2002, 17: 744.

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Zoological Research
2005年 第2期

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