食管鳞状细胞癌及癌前病变组织中p16基因甲基化的研究

CpG island methylation of p16 gene in tissues of esophageal squamous cell carcinoma andits precancerous lesions

目的探讨食管鳞状细胞癌及癌前病变组织p16基因CpG岛的甲基化情况,及其在食管癌发生和早期诊断中的价值。方法采用MSP方法,对食管癌前病变不同阶段、鳞状细胞原位癌和浸润癌的病变组织进行了甲基化检测,并与其相应的正常组织和慢性食管炎组织的甲基化情况进行对比分析。结果轻、中、重度不典型增生、鳞状细胞原位癌和浸润癌的甲基化频率分别为22.73%(5/22)、46.15%(6/13)、77.78%(7/9)、78.57%(11/14)和64.86%(24/37)。67例正常对照组织中3例(4.48%)p16基因甲基化,与病变组织相比,差异有统计学意义,P=0.000。10例慢性食管炎组织中1例(10%)p16基因甲基化。结论p16基因甲基化可能是食管癌发生的最早期事件之一。

OBJECTIVE:To detect CpG island methylation of p16 gene in tissues of esophageal squamous cell carcinoma and its precancerous lesions and to study the role of p16 methylation in the occurrence and early diagnosis of esophageal carcinoma. METHODS: MSP was used to detect 5′-CpG island methylation of p16 gene in esophageal precancerous lesions, carcinoma in situ (CIS) and infiltrated carcinoma of esophageal squamous cell, and comparison analysis was performed in their corresponding normal tissues and chronic esophagitis.RESULTS:The frequency of p16 methylation in mild, moderate and severe dysplasia, CIS and infiltrated carcinoma of esophageal squamous cell were 22.73%(5/22),46.15%(6/13),77.78%(7/9),78.57%(11/14),and 64.86%(~24/37 ), respectively, in 3(4.48%) of the 67 corresponding normal tissues of esophageal lesions and in 1 (10%) of 10 normal tissues,P=0.000.CONCLUSION:p16 methylation may be one of the earlist events in the occurrence of esophageal carcinoma.