血小板膜糖蛋白Ia C_(807)T基因多态性与脑血管疾病易感性的关系研究

Study on the Association of Platelet Glycoprotein Ia C_(807)T Gene Polymorphism with the Susceptibility to Cerebrovascular Disease

目的探讨血小板膜糖蛋白(glycoprotein,GP)IaC807T基因多态性与脑血管疾病遗传易感性的关系及发生机制。方法应用聚合酶链反应-限制性片段长度多态性方法检测139例脑梗死患者、51例脑出血患者及120名正常对照者的GPIaC807T基因型,比浊法检测血小板聚集率。结果年龄≤60岁的脑梗死患者和高危脑梗死患者的T807等位基因频率与正常对照者相比差别有显著性意义(P<0.05)。脑出血组患者C807等位基因频率与正常对照者相比差别无显著性意义(P>0.05)。在对照组与脑梗死组,GPIa基因T等位基因携带者加入胶原90s时血小板聚集率较非T等位基因携带者显著增高(P<0.05),而两者最大血小板聚集率间差别无显著性意义(P>0.05)。结论血小板膜GPIaT807等位基因可能是年轻脑梗死患者和高危脑梗死患者的遗传危险因素,其作用机制可能与血小板聚集功能的迅速启动有关。

Objective To investigate the association of the platelet glycoprotein Ia C 807T gene polymorphism and the genetic susceptibility to cerebrovascular disease and its mechanism.Methods By PCR-restriction fragment length polymorphism technique,the platelet glycoprotein Ia C 807T genotypes were detected in 139 patients with cerebral infarction,51 patients with cerebral hemorrhage and 120 healthy controls,collagen -induced platelet aggregation was measured by turbidimetry.Results The frequencies of GPIa T allele were significantly higher in cerebral infarction group among individuals younger than the mean age of 60 years (χ 2=4.37,P<0.05) and individuals with high-risk factors (χ 2=3.98,P<0.05) than those in the control group.In contrast,no evidence of an association between GPIaC 807T polymorphism with cerebral hemorrhage was found (χ 2=0.07,P>0.05).Collagen-induced platelet aggregation in 90 second was significantly higher in the T allele carriers than non-T allele carriers(cerebral infarction patients and controls),but no significant difference was found in the maximal platelet aggregation between them (P>0.05).Conclusion The Platelet collagen receptor GPIa-IIa T 807 allele might be an independent risk factor for the development of cerebral infarction in younger patients and high-risk patients,and the mechanism may be the rapid initiation of collagen -induced platelet aggregation.