肝病患者红细胞天然免疫黏附功能及红细胞膜CR_1分子测定的临床意义

The Clinical Significance of Detecting Red Cell Nature-Immune-Adhesion Function and Complement Receptor Type1 in Liver Disease

目的观察肝病患者红细胞天然免疫黏附功能(RNIAF)变化及红细胞膜CR1分子数量表达与肝病之间的关系。方法检测30名健康体检者(G组)和确诊的28例急性肝炎患者(A组)、32例慢性肝炎患者(B组)、18例肝硬化患者(C组)、11例肝癌患者(D组)、16例重型肝炎患者(E组)的RNIAF、红细胞膜CR1分子数量表达、血清胆碱酯酶(CHE)和凝血酶原活动度(PTA)。观察各组肝病患者在治疗过程中RNAIF、CR1、CHE、PTA的变化。结果急性肝炎、慢性肝炎、肝硬化、肝癌、重型肝炎组患者的RNIAF及CR1水平显著低于正常对照者(F值分别为17.79、17.28,P均<0.01),而以重型肝炎为著(q值分别为12.57、12.06,P均<0.05)。肝病病情好转者,其RNIAF逐渐回升;病情无好转或恶化者,RNIAF逐渐下降,其敏感性高于CR1、CHE及PTA(P>0.05)。结论RNIAF是肝脏疾病发生、发展和转归的重要参考指标,对临床判断预后有一定参考价值。

Objective To investigate the changes of red cell nature immune adhesion function (RNIAF)and complement receptor type 1( CR 1) on erythrocytes in liver disease.Methods 28cases with acute hepatitis(group A),32 cases with chronic hepatitis (group B),18case with cirrhosis (group C),11cases with hepatocellular cancer (group D),16cases with hepatitis gravis (group E) were studied groups,and 30 healthy blood donors were control group.Suspension of red cells in self-plasma was mixed with solution of mouse carcinoma cells.A tumor cell attached with 5 red cells was counted as one rosette.The adhesion rate was calculated.The CR 1 expressed on erythrocytes were detected by cell-ELISA.The changes of RNIAF,CR 1,CHE,PTA in all patients with hepatitis were regularly detected.Results RNIAF and CR 1 in all studied groups were lower than that in control group (P<0.01).RNIAF was positively correlated CHE and PTA in patients with hepatitis.RINAF was increased earlier than CHE,PTA in the convalescents of hepatitis.Conclusion RINAF is an important judgment line in pathogeny, happenness,development and return prognosis of the liver disease,it has some value in judging prognosis.