显微三维分析软件设计与开发

Study and Development on Three-Dimensional Analysis Software for Microscope

论述了显微三维分析软件系统的组织结构和程序流程; 针对因离焦光线的干扰而产生的模糊光学断层图像序列, 基于断层内相邻像素和断层间相邻像素的相关信息, 采用改进的最大期望算法对光学断层图像序列进行去模糊处理; 对去模糊处理后的三维光学断层图像序列, 介绍了一种基于表面点绘制的三维数据场表面重建反走样方法, 加快了重建速度, 同时具有较好的显示效果; 三维体绘制时, 提出了多维半自动阻光度转换函数解决方案, 清晰地显示出了物体内部的细节变化; 采用基于体素的方法计算重建后的物体的表面积和体积; 通过三维分割和标记算法, 实现了在三维空间内选择感兴趣目标的操作。

This paper deals with the framework and techniques of 3D analysis software for automatic microscope system. Improved expectation maximum image restoration method based on context relationship of pixels of intra-section and inter-section image was present to restore the blurred 3D optical section image series, caused by out-of-focus. An anti-aliasing surface point based rendering technique was proposed for 3D surface reconstruction, the experimental results show that both the surface reconstruction and the display performance of the technique are satisfactory; A multi-dimensional semi-automatic transfer function was chosen for direct volume rending. After 3D reconstruction, the surface area and volume were estimated based on voxel. The system also provided the functions for interactive operation to choose interesting object in 3D space based on 3D segmentation and 3D labeling methods.