摘要
在硅胶G薄层色谱板上,建立了双波长薄层扫描法测定野葛花中葛花苷的分析方法.薄层分析的展开剂为三氯甲烷-甲醇-冰醋酸3∶1∶0.3,葛花苷在0.484～3.872μg范围内呈良好线性.该方法快速、简便,为野葛花作为中药质量控制提供科学依据.
A double wavelength TLC densitometric scanning method to determine kakkalide in Pueraria lobata flowers was established on silica gel chromatoplates. The eluant for the TLC analysis consisted of CHCl 3, CH 3OH and HAc whose proportion was 3∶1∶0.3, and the good linearity appeared when Kakkalide ranged between 0.484 μg and 3.872 μg. This method was quick, simple and convenient, thus providing scientific basis for controlling the quality of Pueraria lobata flowers as a Chinese medicine.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第4期791-793,共3页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省科技攻关计划项目
项目编号2002K11-G85
关键词
野葛化
葛化苷
薄层扫描
Pueraria lobata
kakkalide
TLC scanning
