“副鼻窦冲剂”中川芎嗪的HPLC法测定

High Performance Liquid Chromatographic Assay of Tetramethylpyrazine in“Fubidou Granule”

副鼻窦冲剂其主药为川芎。本文应用HPLC法测定了该制剂中川芎嗪的含量：利用YWG-C_(18)柱，检测波长为292nm，流动相为甲醇-水（52∶48），以无水乙醇溶解样品，过滤后进样。操作简便、快速、准确、重现性好，实验结果：回收率为99．2％，RSD为1．1％（n＝4）最低检测量为0．5μg／ml。

Fubidou Grandle is a new Chinese herbal preparation. It consists of Rizoma Chuanxiong, Fructus Forsythiae and Herba Asari. It is used for the treatment of acute and subacute paran asal sinusitis. This work reports a new HPLC method for the determination of tetramethylpyr azine in the chinese herbal preparation.A revers phase column C18 was used for the separati on with methanol-water (52∶ 48)at 1 .0ml/min as eluentand measured by UV detector at 292nm.The extraction recovery was 99.2％, RSD was 1.1％(n = 4) and the seusitivity limit of quantit ative analysis was 0.5μg/ml. The assayis simple, rapid and sensitive with good reproduction