内毒素血症山羊NO代谢的变化及氨基胍对山羊血浆NO水平的影响

Changes of NO and effect of aminoguanidine on NO level in goats with endotoxemia

为了探讨内毒素(LPS)血症时NO在机体病理损伤中的作用,将22 只山羊随机分为5组,即内毒素血症模型组(LPS 1 800 EU/kg)、内毒素血症氨基胍(AG)组(AG 25 mg/kg)、大剂量内毒素血症模型组(LPS 5 400 EU/kg)和大剂量内毒素血症AG组和对照组。分别于处理后第1、4、8和24 h采集血液,检测血浆中NO水平、环磷酸腺苷活性及血清中一氧化氮合酶活性。结果表明,山羊内毒素处理后一氨化氮合酶活性增强、NO含量增加、环磷酸腺苷浓度升高。提示,内毒素血症时,过量的NO在机体病理损伤中起中介作用,给予诱导型一氧化氮合酶抑制剂AG能缓解内毒素血症进一步恶化。

Twenty-two hybrid goats were divided randomly into experimental Group A, B, C and D, and control group. Group A were administrated with 50mL of saline containing small dose of lipopolysacc- haride (LPS) for 1800EU/kg body weight, Group B were administrated with 25mg of aminoguanidine (AG) after injection of LPS for 1800EU/kg, Group C were treated with 50mL of saline containing large dose of LPS for 5400EU/kg, Group D were treated with 25mg of AG after injection of LPS for 5400(EU/kg), and the control group received 50mL of saline only. The content of serum NO, nitric oxide synthase (NOS) activity and level of cGMP were determined to study NO metabolism. The results indicated that the activity of nitric oxide synthase, and the levels of serum nitric oxide and cGMP increased significantly after LPS injection (P<(0.05)),which suggested that excess NO plays an important role in pathoge- nesis in goats with endotoxemia and AG can inhibit development of endotoxemia.