内皮抑素基因的克隆及其序列测定

Cloning of endostatin gene and its sequencing

内皮抑素(endostatin)能抑制内皮细胞的增殖和转移。试验应用TD PCR法从人胎盘中扩增Endostatin的cDNA片段,将其克隆到pUCm T载体中,并进行克隆基因的序列分析。序列分析表明,该cDNA开放阅读框长555bp,编码184个氨基酸残基的多肽序列,其编码序列与国外报道的相应序列基本一致。

Endostatin has generally proved to inhibit proliferation and migration of endothelial cells. Human endostatin gene fragment was amplified by TD-PCR using human placenta as template and the PCR product was cloned into pUCm-T vector and subjected to DNA sequencing. The cDNA fragment was 555 bp open reading region, which encoded 184 amino acids residues. The coding sequencing of endostaitn is basically consistent to the results reported by foreigner researchers.