蝙蝠葛茎叶抗菌活性成分的研究

Studies on the antimicrobial active constituents of stem and leaves from Menispermum dahuricum DC.

将蝙蝠葛(Menispermum.dahuricumDC.)茎叶的乙醇粗提物分别用石油醚、乙酸乙酯、正丁醇萃取。其萃取物抗菌活性试验结果表明,石油醚萃取物对青霉菌(Penicilliumsp.)有强抑制作用;乙酸乙酯萃取物对假丝酵母菌(Candiasp)有强抑制作用,对金色葡萄球菌(Staphylococcusaureus)和大肠杆菌(Escherichiacoli)有较强抑制作用,对辣椒疫霉菌(Phytophoracapsic)有抑制作用;正丁醇萃取物无明显抗菌活性。经对乙酸乙酯萃取物分离纯化得到2个化合物和,抗菌试验结果表明,化合物具有抑制金色葡萄球菌和辣椒疫霉菌的活性,化合物具有抑制假丝酵母菌和辣椒疫霉菌的活性;经光谱和化学分析鉴定化合物为异槲皮素甙,该化合物为首次从蝙蝠葛植物中得到。

<Abstrcat>The stem and leaves of M.dahuricum DC.were exhaustively extracted with ethanol.The extraction was done with petroleum ether,ethylacetate and n-butanol in turn.The antimicrobial components from various extracts were investigated and found that the components from petroleum ether had high antimicrobial activity on the Penicillium sp.The components from ethylacetate could restrain the Staphylococcus aureus seriously,and have high antimicrobial activity against Candiasp,E.coli and Phytophora capsic.The components from n-butanol had no antimicrobial activity.We derived compound Ⅰ and compound Ⅱ from ethylacetate extract.Compound Ⅰ could restrain the Staphylococcus aureus and Phytophora capsic.Compound Ⅱ had antimicrobial activity on Candiasp and Phytophora capsic.On the basis of spectral data and chemical reaction,Compound Ⅰ was elucidated to be isoquercitrin,which was isolated from the stem and leaves of M.dahuricum DC.for the first time.