荧光探针法研究接枝酪蛋白乳胶粒子形成

Fluorescence probe investigation of internal structure of casein polymerization

在微量铜离子存在的情况下, 用甲基丙烯酸甲酯(MMA) 对酪蛋白进行接枝聚合反应. 用动态激光光散射和荧光探针法研究接枝酪蛋白的乳胶粒子的粒径和内部极性, 实验结果表明, 在接枝聚合反应初期, 以MMA在酪蛋白乳胶粒子内部的接枝共聚反应为主; 进入反应后期, 以MMA自身的均聚反应为主. 当铜离子的浓度增加时, MMA的均聚趋势增强. 与萃取前相比, 萃取后的接枝酪蛋白乳胶粒子的粒径变大, 粒子内部极性增强.

The graft copolymerization of methyl methacrylate (MMA) onto casein was carried out in the presence of Cu^(2+) catalyst. Particle size and inner polarity of the grafted casein latex were investigated via dynamic light scattering and the fluorescence probe method respectively. The dominating reaction at the beginning of grafting copolymerization was MMA grafted onto casein inside of the latex. However the terminal phase was the copolymerization of MMA itself. The homopolymerization of MMA improved with increasing Cu^(2+) concentration. The latex particle size decreased and its inner polarity increased after extract.