两次注血致迟发性脑血管痉挛犬动物模型

A model of experimental delayed cerebral vasospasm in dog with blood injection at twice

本文报告了两次注血致蛛网膜下腔出血（ＳＡＨ）后迟发性脑血管痉挛（ＤＣＶＳ）大的动物模型，两次注血间隔４８小时，两次注血后立即取脑见大量的血液沉积在脑底大血管周围；于第一次注血前与第二次注血后的第五天行椎动脉造影证实ＤＣＶＳ的发生率为１００％，其中６０％重度痉挛；３３．３％中度痉挛；６．７％轻度痉挛。电镜观察证实痉挛动脉管壁各层均存在形态学改变。本实验提示两次注血，致ＤＣＶＳ是一个恒定、可靠、发生率高、操作简便的动物模型。

Abstract This study reports a model of delayed cerebral vasospasm(DCVS) in dogs.Subarachnoid hemorrhage(SAH) and subsequent DCVS were induced by the cisterna magna injections of fresh autoligous blood 48 hours at twice.The dogs were sacrificed 6 days the sacond injection.The brains were observed to identify the large amouns of subarachnoid blood clots around the basilar cisterna of the brain.Angiographic narrowing of the basilar arteries was presented 5 days after the second injection with an incidence of 100%.The severe spasm was in 60%,the moderate spasm in 33.3% and the mild spasm in 6.7%.The spasic basilar arteries showed ultrastructural changes in all layers of the vascular wall with electron microscopic examination.These data suggest that DCVS induced by two cisterns injection is the model of reliability,with high incidence and easy operation.