期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

基质金属蛋白酶-2和纤维粘连蛋白mRNA表达与乳腺癌转移相关性的探讨 被引量：3

The Research on Relativity between the mRNA Expression of MMP-2 and Fibronectin and the Breast Cancer Metastasis
下载PDF
导出
摘要 目的:探讨基质金属蛋白酶-2(MMP-2)和纤维粘连蛋白(Fibronectin,FN)mRNA表达量与乳腺癌转移的关系。方法:采用荧光定量RT-PCR方法检测30例乳腺原发癌和配对淋巴结转移癌的MMP-2和FNmRNA表达量,分析MMP-2和FNmRNA表达量与乳腺癌转移的关系。结果:30例淋巴结转移癌的MMP-2和FNmRNA表达量低于原发癌,组间比较差异有显著性(P<0.05)。MMP-2和FNmRNA表达量呈线性分布,但线性回归和双变量线性相关分析表明两个基因的mRNA表达量无相关性(P<0.05)。结论:MMP-2和FNmRNA表达量在转移癌较原发癌下调。 Objective: To discuss the relationship and clinical significance in breast cancer and mRNA expression of matrix metalloproteinase-2 (MMP-2) and Fibronectin. Methods: Detect MMP-2 and Fibronectin mRNA expression in 30 cases of primary breast cancer and paired lymphnode metastasis by flurence-quantitative RT-PCR and analyze the relativity and clinical siganificance of MMP-2 and Fibronectin mRNA expression with breast cancer metastasis. Results: The MMP-2 and Fibronectin mRNA expression of 30 paired lymphnode metastasis are lower than that of primary breast cancer. The difference is obvious(P<0.05). The MMP-2 and Fibronectin mRNA expression is linear diffusion, but there is no liner relativity between the MMP-2 mRNA expression and Fibronectin mRNA expression (P<0.05). Conclusion: The MMP-2 and Fibronectin mRNA expressions are low-regulated in metastatic carcinoma than in primary cancer.
作者 肖春花 冯玉梅 李晓青 郝希山
机构地区 天津医科大学附属肿瘤医院
出处 《中国肿瘤临床》 CAS CSCD 北大核心 2005年第8期424-427,共4页 Chinese Journal of Clinical Oncology
基金 天津市科技发展计划基金资助(编号:013182311)
关键词 乳腺癌 转移 基质金属蛋白酶-2纤维粘连蛋白 Breast cancer Metastasis Matrix metalloproteinase-2 (MMP-2) Fibronectin (FN)
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1PO van Trappen, ARyan, M Carrol, et al. A model for co-expression pattern analysis of genes implicated in angiogenesis and tumor cell invasion in cervical cancer[J]. BrJ Cancer, 2002, 87(6):5337～544.
  • 2Liotta LA, Steeg PS, Stevensen WG, et al. Cancer metastasis and angiogenesis: An imbalance of and negative regulation [J]. Cell,1991, 64(2): 327～336.
  • 3Erik W Thompson, Ming Yu, Jorge Bueno, et al. Collagen induced MMP-2 activation in human breast cancer[]]. Breast Cancer Res Treat, 1994, 31(2/3): 357～370.
  • 4Massova I, Kotra LP, Fridman R, et al. Matrixmetalloproteinases:structures, evolution, and diversification [J]. FASEB J, 1998, 12:1075～1095.
  • 5Anne Kallioniemi. Molecular signatures of breast cancer-predicting the future [J]. New EngJ Med, 2002, 347(25): 2067～2068.
  • 6Liao YF, Gotwals PJ, Koteliansky VE, et al. The E Ⅲ A segment of fibronectin is a ligand for integrins providing a novel mechanism for regulation cell adhesion by alternative splicing [J]. J Bio Chem, 2002, 277(17): 14467～14474.
  • 7Kosmehl H, Berndt A, Katenkamp D, et al. Molecular variants of fibronectin and laminin: structure, physiological occurrence and lhistopathological aspects [J]. Virchows Arch, 1996, 429 (6): 311 ～322.
  • 8Davidson B, Goldberg I, KopolovicJ, et al. MMP-2 and TIMP-2 expression correlates with poor prognosis in cervical carcinoma-a clinicopathologic study using immunohistochemistry and mRNA in situ hybridization [J]. Gynecol Oncol, 1999, 73 (3):372～382.
  • 9邱志强,孙保存,张诗武,赵秀兰,郝希山.乳腺癌转移相关基因表达蛋白组织微阵列的初步研究[J].中国肿瘤临床,2004,31(5):252-255. 被引量：8
  • 10Xishan H, Baocun S, Wei ZH, et al. Differential gene and protein expression in primary breast malignancies and their lymph node metastases as revealed by combined cDNA microarray and tissue microarray analysis[J]. Cancer, 2004, 100(6): 1110 ～ 1122.

二级参考文献9

  • 1[1]Lawrence B, Anthony G, Stuart S, et al. Vascular stroma formation in carcinoma in situ, invasive carcinoma, and metastatic carcinoma of breast[J]. Clin cancer Res, 1999, 5(5):1041 ～ 1056
  • 2[2]Ase Bratland, Karianne R, Gunhild M, et al. Expression of a novel factor, coml., is regulated by 1,25-dihydroxyvitamin D3 in breast cancer cells[J]. Cancer Res, 2000, 60(19):5578～5583
  • 3[3]Silvia F, Juan F, Dennis S, et al. Expression of metastases associated genes in cervical cancers resected in the proliferative and secretory phases of the menstrual cycle [J]. Clin Cancer Res, 2000,6(12):4653～4657
  • 4[4]Hiro-omi K, Kinoya Y, Yasushi K, et al. Prognostic value matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in bladder cancer[J]. Cancer, 1998, 82(7):1359 ～ 1366
  • 5[5]Liu S, Sarah Netzel-Arnett, Henning Birkedal-Hansen, et al. Tumor cell-selective cytotoxicity of matrix metalloproteinase-activated anthrax toxin[J]. Cancer Res, 2000, 60(21):6061～6067
  • 6[6]Yang GY, Zhang ZH, Liao J, et al. Immunohistochemical studies on waf1/P21WAF1,p16, pRb and p53 in human esophageal carcinomas and neighboring epithelia from a high-risk area in Northern China[J]. IntJ Cancer, 1997, 72(5):67
  • 7[7]Leivonen M, Nording S, Lundin J, et al. P27Kipl expression correlates with short-term, but not with long-term prognosis in breast cancer[J]. Breast Cancer Res Treat., 2001, 67(1):15～22
  • 8[8]Akano Y, Kato Y, Paul J, et al. cyclinD1 overexpression and lack of P27Kipl correlate positively and cyclin E with poor prognosis in gastric cancer [J]. American Journal Pathology, 2000, 156(2):585 ～ 594
  • 9[9]Weinstein IB. Disorders in cell circuitry during multistage carcinomagenesis: the role of homeostasis [J]. Carcinogenesis, 2000, 21(5) :857 ～ 864

共引文献7

同被引文献14

  • 1胡卫东,林从尧,熊斌,李春光,胡名柏.腋淋巴结阴性乳腺癌外周血hMAM检测意义[J].中国肿瘤临床,2005,32(12):674-677. 被引量：6
  • 2刘剑仑,杨华伟,曹骥,杨南武.乳腺癌hMAM mRNA和CD44V6 mRNA表达及临床意义[J].肿瘤,2005,25(6):581-584. 被引量：3
  • 3李树玲.乳腺癌的早期诊断[J].中国肿瘤临床,1988,15(2):105-105.
  • 4金显宅主编.乳腺癌的研究.天津:学苑出版社.1985.240-245.
  • 5Schneeweiss A, Sinn HP, Ehenmann V, et al. Centrosom aberrations in primary invasive breast cancer are associated with nodal status and hormone receptor expression[J]. Int J Cancer,2003,107(3):346- 352.
  • 6Lason RN, et al. Early Breast Cancer [J].Can Med Assoc,1956,75:309.
  • 7Sis B, Sa ol O, Kiipelio lu A, et al. Prognostic significance of matrix metalloproteinase-2, cathepsin D, and tenascin-C expres- sion in colorectal carcinoma[J]. Pathol Res Pract, 2004, 200(5): 379--387.
  • 8Moran A, Iniesta P, Garcia-Aranda C, et al. Clinical relevance of MMP-9, MMP-2, TIMP-1 and TIMP-2 in colorectal cancer[J].Oncol Rep, 2005, 13(1): 115-120.
  • 9Redondo P, Lloret P, Idoate M, et al. Expression and serum levels of MMP-2 and MMP--9 during human melanoma progression[]]. CAin Exp Dermatol, 2005,30(5): 541-545.
  • 10Tutton MG, Sharif SP, Eccles SA, et al. Preoperative plasma MMP-2 expression is prognostic in colorectal cancer[J]. Colorectal Disease. 2006, 8(8): 722-724.

引证文献3

二级引证文献4

中国肿瘤临床
2005年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部