摘要
目的对合成的ICAM-1模拟肽P1(KLYLIAEGSVAA)的抗原性及生物活性进行鉴定。方法化学合成ICAM-1模拟肽KLYLIAEGSVAA,以间接ELISA及竞争抑制试验鉴定合成肽的抗原性,以玻片免疫组化方法鉴定合成肽的生物活性。结果合成肽能与抗ICAM-1单抗15.2发生特异性结合,并且能够竞争抑制ICAM-1分子及相应的阳性噬菌体克隆与单抗15.2的结合作用。玻片免疫组化显示合成肽及ICAM-1分子均能有效抑制相应的阳性噬菌体展示肽与白细胞的结合。结论合成的短肽具有ICAM-1与15.2抗体结合的抗原性,并能有效模拟ICAM-1分子与白细胞结合的功能。
Objective To identify the antigenicity and bioactivity of synthetic peptide P1(KLYLIAEGSVAA). Methods ICAM 1 mimicry peptide P1 was synthesized and its immunoreactivity was identified by indirect ELISA and competitive suppressive ELISA. Its bioactivity was detected by immunohistochemical staining. Results The peptide was found to reactional with anti ICAM 1 monoclonal antibody 15.2, and it inhibited, through competition, the binding of 15.2 antibody to immobilized ICAM 1 and the binding of the corresponding positive phage clones to the immobilized 15.2 antibody. Also the peptide completely blocked the interaction between phage borne peptide with the immobilized LFA 1 expressing leukocytes. Conclusion The synthetic peptide KLYLIAEGSVAA showed similar immunoreactivity and bioactivity to ICAM 1, which might be an useful short peptide analog of ICAM 1.
出处
《热带医学杂志》
CAS
2005年第1期5-7,共3页
Journal of Tropical Medicine
基金
国家自然科学基金(No.39800126)。