p53、CD34与nm23在肝癌诊断及临床预后中的作用

The Significance of Expression of P53,nm23 and CD34 in the Diagnosis and Prognosis of Hepatocellular Carcinoma

目的探讨p53、CD34和nm23基因蛋白表达在肝癌诊断及临床预后中的意义。方法用免疫组化SP法检测50例肝癌组织和55例肝非癌性增生结节p53、nm23、CD34的表达,分析它们与肝癌临床病理特征的相关性。结果p53、CD34在肝癌组织的表达比非癌组明显增高,且在肝癌组中伴有与不伴门脉瘤栓的两组间有统计学差别(P<0.05)。nm23则在非癌组中表达率高,而且nm23在癌组织中的表达与显著的瘤周淋巴细胞浸润有关(P<0.05)。不同蛋白组合联合分析生存情况发现:P53(+)、CD34(+)、nm23(-)组同其他组相比,生存时间明显为短(P<0.05)。结论p53、nm23、CD34的综合表达对肝癌有重要的辅助诊断价值,并且p53突变、CD34的高表达似乎可作为肝癌预后不良的可行性指标。

Objective To explore the significance of expre ssion of P53,nm23and CD34in the diag nosis and prognosis of hepatocellular carcin oma.Methods Immunohistochemical technique was adopted to detect the expression levels of p53,nm23and CD34in 50cases of HCC.The prognosis was analyzed accordi ng to the clinicopathological featu re indexes,which living time less than12months is considered as poor prognosis,more than12months as goodprognosis,an d used 55cases of non-neoplastic liver tissue and ben ign lesions as inner contrast.Results The expression of P53,CD34was higher in the HCC group than in the inner group,and their expression was associated with portal vein embolus(P<0.05).The expression of nm23was higher in the non-neoplastic liver tissue than in the HCC group,a nd nm23was associated with prognosis and prominent peritumoral lymphocytic infiltration(P<0.05).Combined analysis revealed that P53(+),CD34(+),nm23(-)groups had a poor prognosis than the o thers.Conclusions Detection of P53,nm23and CD34may be very useful to HCC diagnosis and prognosis.And the ove r expression of P53,CD34are considered acceptable as the parameters in p redicting of poor prognosis in HCC.