土槿甲酸对3种细胞系增殖抑制作用的比较

Effect of pseudolaric acid A on inhibition proliferation of three cell lines

目的比较土槿甲酸(PAA)对人胃癌细胞(MGC80-3),人肝癌细胞(SMMC-7721)和人红白血病细胞(K562)的增殖抑制作用,探讨其抗癌作用机制。方法采用MTT法检测不同浓度PAA对体外培养的MGC80-3细胞,SMMC-7721细胞和K562细胞的细胞毒作用。Hoechst33342/PI荧光双染色方法检测细胞凋亡。琼脂糖凝胶电泳检测K562细胞DNA裂解。结果PAA明显抑制MGC80-3,SMMC-7721和K562细胞系的增殖,其IC50值分别为12.500,3.125,12.500μmol·L-1。凋亡细胞比例在用药48h分别达83.50%,44.50%和63.20%。琼脂糖凝胶电泳呈典型的"DNALadder"。结论PAA能有效抑制MGC80-3,SMMC-7721和K562细胞增殖并诱导凋亡。

OBJECTIVE: To study effect and mechanism of PAA on tumor cell line MGC80-3, SMMC-7721 and K562. METHODS: PAA in different concentrations was added into the medium in which the cells were cultured. Cytotoxicity rate was detected by MTT assay. The apoptosis was examined by Hoechst 33342/PI staining and DNA agarose gel electrophoresis. RESULTS: The cell growth was remarkably inhibited by PAAJC50 values of PAA for MGC80-3, SMMC-7721 and K562 cell line were 12.500,3.125 and 12.500 μmol &middot L-1 respectively. The apoptotic rate was respectively 83.50%, 44.50% and 63.20% after 48 h of exposure to PAA. DNA ladder appeared on 1.5% agarose gel electrophoresis. CONCLUSION: PAA efficiently induced growth inhibition and apoptosis in MGC80-3, SMMC-7721 and K562 cell lines.