F344大鼠膀胱肿瘤模型的制备与检测

The examination of experimental model of bladder tumors in F344 rats induced by MNU

目的通过膀胱灌注N-甲基亚硝基脲(MNU)诱导F344大鼠膀胱肿瘤模型。方法选取F344大鼠40只,随机分为两组第1组膀胱灌注生理盐水(对照组),第2组膀胱灌注MNU(MNU组),每2周1次,共5次;于第14周处死动物,取血清测干扰素(INF-γ),膀胱肿瘤组织测主要组织相容性抗原-I(MHC-I)及病理观察。结果MNU组出现膀胱肿瘤,对照组无1例肿瘤;对照组血清INF-γ为(9.620±7.256)pg/ml,实验组为(0.898±0.323)pg/ml,两组相比有统计学意义(P<0.001)。实验组膀胱肿瘤的MHC-I表达较高(58.60%～93.80%)。结论MNU可成功诱导F344大鼠膀胱肿瘤,该模型是肿瘤免疫治疗的理想模型。

Objective To establish the model of bladder tumors in F344 rats by N-methyl-N-nitrosourea (MNU) intravesically. Methods Twenty F344 rats (group A) received 0.1 ml 0.9%NaCl intravesically and the other twenty ones (group B) received 0.1 ml 20 mg/ml N-methyl-N-nitrosourea intravesically every other week (weeks 0, 2, 4, 6 and 8) for a total of five times. After week 14, rats were executed. Their INF-γ of blood serum was examined by ELISA and the MHC-I of bladder tumors was examined by FCM. Results Everyone in group A was normal, but everyone in group B had bladder tumor. INF-γ of blood serum in group A was (9.620±7.256) pg/ml, while it in group B was (0.898±0.323) pg/ml (P<0.001). MHC-I of bladder tumors in group B was expressed very high (58.60%～93.80%). Conclusion The bladder tumors' model in F344 rats could be induced by MNU intravesically. The model was very ideal for immunotherapy of cancers.