小鼠进展性肾病模型建立的探讨及相关研究

Establishment of model for chronic nephropathy in mice

目的为了更好地研究慢性进展性肾病的发病机制,寻求建立一个与人类慢性肾病极其相似的动物模型。方法给予Balb/c小鼠尾静脉注射阿霉素10～11mg/kg后观察0,1,2,4,6,8周体重、尿蛋白、血清白蛋白、血甘油三酯、胆固醇、肾功能、外周血T淋巴细胞及病理学改变。结果小鼠慢性进展性肾病模型复制成功。尿蛋白、血甘油三酯、胆固醇随着实验的进程明显增高,而血清白蛋白、肾功能进行性下降,肾脏病理由微小病变型发展至肾小球硬化型。结论本实验成功地建立了一个稳定性好、重复性好、个体差异小且与人类慢性肾病极其相似的小鼠慢性进展性肾病模型,为更好地研究慢性进展性肾病的发病机制提供了保证。

Objective To establish model of chronic nephropathy in mice which is similar with human nephropathy for research of the pathogenesis. Methods Male Balb/c mice were intravenously injected with a single dose of ADR(10.5 mg/kg). Five mice were sacrificed at 0, 1st, 2nd, 4th, 6th, 8th week and determined 24 h urinary protein, serum albumin, serum triglyceride, cholesterol,renal function, peripheral T lymphocytes and pathology were observed. Results The model of chronic nephropathy in mice succeeded. Over proteinuria,serum triglyceride, cholesterol elevated and serum albumin, renal function decreased throughout the study period, the minimal change nephropathy developed to glomerulosclerosis. Conclusion The model should be useful in unraveling the pathogenesis of golmerular and interstitial inflammation and fibrosis in chronic renal disease.