抗DNA酶B和抗链“O”在风湿热诊断中的应用

The Application of Anti-Dnase B and Antistreptolysin “O”Microtitration Test in the Diagnosis of Acute Rheumatic Fever

目的:评估抗DNA酶B和抗链“O”(ASO)在溶血性链球菌感染的血清学检测对风湿热的诊断价值。方法:抗DNA酶B用微量法,ASO用速率散射比浊法,测定77例急性风湿热患者,35例活动期风湿性心脏病患者,5 0例对照组。结果:急性风湿热组ASO阳性率76 .6 % ,与抗DNA酶B71.4 %差异无显著性(χ2 =0 .8310 ,P>0 .0 5 ) ,二者同时检测阳性率为92 .2 % ,显著高于单一检测(χ2分别为7.10 77、12 .2 775 ,P<0 .0 5 ;活动期风湿性心脏病ASO阳性率5 4 .3%显著低于抗DNA酶B85 .7%的阳性率(χ2 =2 0 .16 ,P<0 .0 1,二者同时检测阳性率可达91.4 % ,显著高于单纯ASO检测(χ2 =0 .12 75 ,P>0 .0 5 )。结论:ASO和抗DNA酶B联合检测可提高诊断阳性率,对风湿热的诊断具有重要的临床价值。

Objective:To evaluate the diagnostic values of anti-DNase B and Antistreptolysin “O”(ASO) microtitration test in diagnosis of acute rheumatic fever with group A streptococcal(GAS) infection.Methods:Anti-DNase B antibodies of 77 patients with acute rheumatic fever(ARF) and 35 acute rheumatic heart disease(ARHD) patients with rheumatic activity ARHD were detected with anti-Dnase B microtitration test and ASO with rate scatter nephelometry test. 50 cases of normal persons were regarded as the control group.Results:The positive rate of ASO was 76.6% and the positive rate of anti-DNase B was 71.4% ,and There were no difference between them. The positive rate of either anti-DNase B and ASO detected jointly were 92.2%;There were significant differences in the positive rate between jointly and singly. The positive rate of anti-DNase B (85.7%) was significantly higher than that of ASO(54.3%) in ARHD, the positive rate of anti-DNase B and ASO were 91.4%, and significantly higher than that of ASO detected.Conclusion:Detecting jointly anti-DNase B and ASO should be of important value in the diagnosis of ARF.