葛根超微饮片的HPLC指纹图谱研究

HPLC fingerprint of Radix Puerariae

目的 研究葛根超微饮片的HPLC指纹图谱, 考察其有效成分及含量, 为葛根的质量控制提供可靠方法。方法 以大连依利特公司Hypersil BDS C18 (4. 6 mm×200 mm, 5μm) 柱为色谱柱。以乙腈水为流动相进行二元梯度洗脱, 柱温为25 ℃, 流速为1. 0 mL·min-1, 检测波长为250 nm。结果 初步建立了葛根超微饮片的HPLC指纹图谱, 标定了11个共有峰。结论 利用HPLC指纹图谱可以较全面的控制葛根超微饮片的内在质量。

OBJECTIVE To determine the HPLC fingerprint of Radix Puerariae and to test its potent constituents and content.METHODS Chromatographic column:hypersil BDS C_ 18(4.6 mm×200 mm,5 μm).Mobile phase:acetonitrile and water in a gradient mode.Column temperature:25 ℃.Flow rate:1.0 mL·min -1.Wavelength:250 nm.RESULTS The fingerprint of Radix Puerariae was established and 11 common peaks were found in the fingerprint.CONCLUSIONS The methods and data can be used to control the quality of Radix Puerariae.