侵染新疆甜菜的两种病毒分离物的研究

The Study on Two Virus Isolates of Beet in Xinjiang

在新疆甜菜的根部和叶片分离到两个球状病毒分离物。从病毒形态、生物学及物化性质等研究结果证明这两个分离物实是一种病毒。病毒粒体力２０面体，直径约２８ｎｍ，回接到甜菜上产生环斑和沿脉线纹最后形成坏死斑。此外还侵染苋色藜，昆诺阿黎，番杏，菠菜等，引起局部坏死斑。在普通烟，心叶烟，菜豆，黄瓜，蕃茄上无症状侵染，病毒致死温度为７０℃，１０分钟。体外存括期１３天以上，冻干病叶７年后仍有侵染力。通过ＰＥＧ沉淀、差速离心、琼脂糖柱层析及蔗糖梯度离心，可得到较高浓度的纯化病毒，病毒的紫外吸收最低值在２４５ｎｍ，最高值在２６０ｎｍ。经ＳＤＳ－ＰＡＧＥ测定，病毒外壳蛋白的分子量为２．７×１０￣４道尔顿，病毒基因组核酸为三个组份，分子量分别为３．０８ｋｂ，１．２８ｋｂ和０．８５ｋｂ。在琼脂双扩散实验中，能与番茄黑环病毒（ＴＢＲＶ）抗血清产生较弱的沉淀线，与黄瓜花叶病毒（ＣＷＶ）、烟草环斑病毒（ＴＲＳＶ）、烟草坏死病毒（ＴＮＶ）、香石竹环斑病毒（ＣａＲＳＶ）的抗血清不发生反应。

wo virus isolates were found in beet in Xinjiang.After they are inoculated to beet the isolates induced yellow ring spot and line patterns along vein of leave.The rmgspot and line patterns eventuallyformed necrosis.The virus infected Chenopodium amaranticolor C.quinoa, Telragnia axpansa and Spinacia oleracea and induced local necrotic leeions.The virus infected Nicltiana tabacum,N. glolionosa,Pelunia hy brida,bean,cucumber and tomato but without showing symptoms.The virus particles have icosahedramorphology with an averege diameter of 28nm,In sap the virus thermal inactivation point(10min)is70℃.Infectivity is retained for 13 days at room temperatue about 20℃ and for 7 years in refrigerat ed dry leaves.The highly purified virus is easily obtained from C.amaranticolor by precipitation withPEG and differential centrifugation,Absorption spetrum of the virus is maximum in 260nm and minimum in 245nm.Protein subunits have M,W.27000.Cenome nucleic acid of the virus consists of three species with sizes of 3.08kb,1.28kb and 0.85kb,respectively,In the agar-double-diffusiontest,the virus reacted tomoto black ring virus antiserum,the faintly precipitation lineis observed, but we did not see reaction of the viurs to antiserum of tabacco ring spot virus,carnation ring spot virus,tabacco necrotic virus,cucumber moseic virus.