乙型肝炎病人尿细胞中HBV的实验研究

Detection of HBsAg,HBeAg and HBV DNA in Urinary cellsof Patients with Hepatitis B and HBsAg Carriers

本文采用间接免疫荧光法（ＩＦ），ＲＰＨＡ法，ＥＬＩＳＡ法及斑点杂交技术检测１０例无症状ＨＢ－ｓＡｇ携带者及８９例乙肝病人尿细胞中的ＨＢｓＡｇ、ＨＢｅＡｇ及ＨＢＶＤＮＡ，发现尿细胞中有ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢＶＤＮＡ存在。结果提示：乙肝无症状携带者及乙肝病人尿细胞中具有ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢＶＤＮＡ，因此更进一步证实尿液具有传染性。

his paper reports the detection of HBsAg,HBeAg and HBV DNA in the urinary cells by IFARPHA ELISA and molecular hybridization techniques.10 HBsAg carriers and 89 patients with hepotitis B were exanlined.The results indicated that HBsAg,HBeAg and HBV DNA could be detected inthe urinary cells.It also susgests that the urine of carriers and patients with hepatitis B be infectious.