用质量控制狭缝式杂交法（Coslot）检测妇女宫颈上皮中人乳头瘤病毒（HPV）DNA

A New Method for the Study of HPV Molecular Epidemiology - Quality Control Slot Hybridization(Coslot)

本文报道一种能对ＨＰＶ进行较为准确分型的刚Ａ杂交程序和方法。它具有如下特点：１．由于简化了实验程序，标本消耗减少。因此该方法尤其适用于小组织活检标本（１－２ｍｇ）中ＤＮＡ同源序列检测；２．敏感性高。由于标本消耗减少，使ＨＰＶ检出率大为提高，与斑点杂交技术相比，敏感性提高１．６倍；３．型别特异性强。由于改变了实验条件及在实验过程中实行严格的质量控制，明显减少了不同ＨＰＶ型别的交叉反应，甚至在部分出现交叉反应的标本中，仍可通过强度对比来最后判定ＨＰＶ感染型别。应用该技术对１４３份宫颈炎标本、１７份宫颈不典型增生标本及２５份宫颈癌标本进行了ＨＰＶ１６型检测，阳性率分别为５０．０５％（７３／１４３）、５７％（２７／４７）及７６％（１９／２５），并与Ｄｏｔｂｌｏｔ方法进行了比较。该方法在ＨＰＶ感染与宫颈癌关系的分子流行病学研究中具有重要意义和广阔的应用前景。

he infection of HPV is the most frequent among the viruses tropic to the epithelium of cervix.The different HPV types possess various infective location and the abilities of etiology,for example,HPV type 6B,type 11 were correlated with the cervical non-tumor lesions,otherwise,the HPV type16 and type 18 were clasely assoctated with cervical carcinoma,so that it is important to identify accu-rately HPV type in the moledular epidemiological researchment for the relationship between cervicalHPv infection and the development of cervical carcinoma.We reperted here a new DNA hybridization method and procedure,with which we can easily differ one HPV type from another.The advantages as follow compared with other hybridimtion method.1.It lreer,mes sllitable to detect small pieces of biopeies from cervix since the experiment procedure being simplified and specimens consuption being decreased;2.The sensitivity is increased by 1.68 foldcomparing with dot blot hybridization;3.It has a higher types specificity owing to medifying the ex-periment condition and putting quality control into the whole experiment procedure.The result showedthat the crossing hybridization among various HPV types was decreased greatly and HPV typing maybe identified according to comparing intensities on the X-ray film even in part of crossing reaction sam-ples.The method may be important and very useful in diagnosis of the HPV infection for cervix,especially in large scale molecular epidemiological investigation for the association of HPV infection withthe cervical carcinoma.