生物素标记HBV RNA探针的制备及应用

Preparation of Biotin HBV RNA Probe andIts Appllcation in Serum Detection

本文首次采用ＳＰ＿（６５）特殊质粒与人类的乙型肝类病毒ＤＮＡ（ＨＢＶＤＮＡ）重组，制备了Ｂｉｏ－ＨＢＶＲＮＡ探针，能特异地与ＨＢＶＤＮＡ杂交。将该探针与缺口转移（ＮｉｃＨｒａｎａｌａｔｉＯｎ）方法标记的ｍ小ＨＢＶＤＮＡ探针进行了比较。结果显示出Ｂｉｏ－ＨＢＶＲＮＡ探针比Ｂｉｏ－ＨＢＶＤＮＡ探针的敏感性提高１０倍。并分别应用两种探针同时检测７０例己肝病人血清中ＨＢＶＤＮＡ，阳性率各为３１．４２％、２８．５７％（Ｐ＞０．２５）．对Ｂｉｏ－ＨＢＶＲＮＡ探针应用于临床检测乙肝病毒ＤＮＡ的效能也进行了初步探讨。

epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP_(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection of HBV DNA,HBVRNA probe are 10 times sensitive than that of HBV DNA.In addition,70 serum saniples of hepatitis B potients have been detected by hybridization withtwo probes.Pooitive proportion of HBV DNA corresponils to 31.42%, 28.57%(P>0.25).The clinical application of HBV RNA probes was discusseil preluninarily.