HBV－DNA的PCR二步法扩增及其快速检测

Researches on Two-step PCR Amplification and a Rapid Detection of HBV-DNA

ＨＢＶ－ＤＮＡ的ＰＣＲ二步法扩增及其快速检测方勤，吴云涛，蔡宜权（中国科学院武汉病毒研究所，武汉４３００７１）关键词ＨＢＶ－ＤＮＡ，二步温控ＰＣＲ，Ｓｏｕｔｈｅｒｎ杂交，生物素寡聚核苷酸杂交乙型肝炎是危害人类健康的主要疾病之一，其病原常用的检测方法多...

specific,sensitive,fast and convenient HBV-DNA detection system has been developed by usin8 two-step PCR combining with a biotinylated oligonucleotide hybridization method.The specificityof the system was proved by hybridizing both ￣(32)P-labelled HBV-DNA and biotinylated oligonucleotideprobes with PCR preduets,We also explored the sensitivity of the system,which can detect as less asabout 1-2 HBV-DNA molecules.The convenience of the system was demonstrated by the simple twotemperature PCR stepe and fast preducts detection with biotinylated oliunucleotide probe,This systemcan be used for both labortltory and clinical detection of HBV-DNA.