PCR在慢性宫颈炎HSV感染研究中的应用

Use of Polymerase Chain Reaction for the Infection with Herpes Simplex Virus in Chronic Cervicitis

ＰＣＲ在慢性宫颈炎ＨＳＶ感染研究中的应用蔡红，姚，季晓辉，周瑶玺（南京医科大学微生物学教研室，南京２１００２９）关键词多聚酶链反应，单纯疱疹病毒，宫颈炎单纯疱疹病毒（ＨＳＶ）被认为与宫颈癌有关。目前生殖道ＨＳＶ感染日渐升高，日益引起人们的重视。由于药...

CR was adapted to detect HSV-DNA sequence in samples from 72 petients with clironic cervici-tis and 30 healthy cotrols,and compared with McAb-ELISE and virus isolation(VI).The plsitive rateof HSVby these three methods was 28.4% 22.5% 20.6% respotively,and coinciuent rates a-mong threelmired methforwere allover 90%,It isshown that PCR cpmbined withcell lysis isa fast,effective and relle ble methed to detect HSV in the cervix,It can detect not only very low quantityHSV-DNA,but also inactive and latent HSV.HSV postivity rate of chronic cervicitis is markedly high-er than healthy control(P<0.05).