犬传染性肝炎病毒在体外细胞质内的发生

Cytoplasmic Morphogenesis of Infectious Canine Hepatitis Virus in Dog Kidney Cells

通过对犬传染性肝炎病毒（ＩＣＨＶ）在犬肾传代细胞内形态发生及其抗原定位的电镜和免疫胶体金电镜研究，发现ＩＣＨＶ除了在宿主细胞核内发生外，还有一条细胞质内的发生途径。在细胞质内病毒核壳体的装配是以均质致密包涵体和副晶格包涵体为“基地”，这与人们熟知的细胞核内形态发生方式相似。免疫胶体金标记显示，细胞质包涵体中含有大量的ＩＣＨＶ抗原成分，显核壳体在细胞质内装配病毒的结构蛋白来源。此外，在感染的细胞质内还观察到与核内相同的病毒核心样结构。

he morphogenesis and antigen localization of infectious canine hepatitis virus(ICHV)in dog kidney cells were studied with electron microscopy and immunogold electron microscopy,Apart from theassembly of ICHV in nuclei,virions were also assembled in cytoplasm of the infected cells.The modeof viral morphogenesis in homogeneous dense inclusions and paracrystalline ones in cytoplasm was similar to that well known in nuclei.Result of immunogold labelling showedthathere there were a lot of anti-genic components of ICHV providing the viral structural protein for the assembly of nucleocapsids inthese two kinds of inclusions in cytoplasm,In addition,some virus core-like electron opaque structuresaround 50nm in diameter were presented in cytoplasm as those in nuclei.