宁夏甜菜的烟草环斑病毒的鉴定

Identification on Tobacco Ringspot Virus of Sugar Beet in Ningxia

从宁夏患丛根病甜菜的病根中分离到一种球状病毒粒子，直径约为２８ｎｍ。提纯病毒的紫外扫描呈典型的核蛋白曲线，最大吸收为２６０ｎｍ，最小吸收为２４０ｎｍ，Ａ＿（２６０）／Ａ＿（２８０）＝１．３０。寄主范围广，能侵染茄科、豆科、藜科、葫芦科、番杏科等１７种植物。病毒的ＴＤＰ为６５℃，ＤＥＰ为１０￣（－３），体外存活期６－７天。有较强的免疫原性。病毒的抗血清与烟草环斑病毒（ＴＲＳＶ）之间产生明显的沉淀线。经初步鉴定认为该分离物是烟草环斑病毒。

sphere-sliaped virus has been isolated from the diseased sugar beet in Nighxia,The virus parti cle was 28nm in diameter.The virus could infect seventeen plants,They belong Cenopodiaceae,Legu minosae,Cucurbitaceae,Solanaceae and Aizoaceae,respectively,There has serological relationship be tween this virus and TRSV.On the basis of the symptomes in different host plants morphological structure of the virus particle and serological test,we preliminarily conclude that the virus belongs to a strain of the Tobacco ringspot virus.