CM_2细胞株及其类C型逆转录病毒的电镜观察

Eoectron Microscopic Study on CM_2 Cell Line and Its Harbored Type C-like Retrovirus

本文报告了ＣＭ＿２细胞的超微结构及其所携带的Ｃ型病毒颗粒形态和形态发生。细胞大小约７－１５μｍ，细胞及其核的多形性具有恶性淋巴细胞特征。在细胞外和细胞内扩张的内质网地中可见大量Ｃ型病毒颗粒。病毒颗粒大小约６８．１－９４．３ｎｍ，平均８１．２ｎｍ。细胞外的病毒颗粒多数具有大小、形态各异的致密核芯，而在内质网池中病毒颗粒的核芯多数呈低密度。病毒颗粒从细胞膜通过芽生方式形成。

he ultrastructure of CM_2 cell line,derived from peripheral blood of a patient with nervous syatem disease and the morphology and morphogenesis of its harbored type C virions were reported.Thecell size by electron micrcacopy in thin section was about 7-15 micron in diameter.The pleomorphiccells have markedly deforined nuclei and the feature of malignant lymphoblast.There were numeroustype C virions in the enlarged cistern of rough endoplasmic reticulum(RER)of the cell and out of thecell.The size of the virion was about 68.1-94.3 nanometer in diameter,in average about 81.2nanometer,The majority of virions out the cell had dense core,but the core of the virions in the RERcistern were with much less density,Virions were formed by budding at cell membrane.