豆科植物上分离的黄瓜花叶病毒（CMV）五个分离物的比较研究

Comparative Studies on Five CMV Isolates from Leguminous Crops

从豌豆、扁豆、菜豆和赤豆分离到五个ＣＭＶ分离物，除寄主反应及核酸组分外，５个分离物在体外抗性、蚜虫传毒、提纯病毒粒体形态和稳定性、衣壳蛋白分子过和病毒粒体迁移率等方面无明显差异．在琼脂糖双扩散试验中五个分离物与ＣＭＶ抗血清形成的沉淀线相互融合，在Ａ蛋白双抗体夹心ＥＬＢＡ测定中，也无明显差异。根据在四种豆科植物上的症状反应，五个分离物可归为两个型：Ⅰ型在豌豆、蚕豆、菜豆和豇豆上产生局部枯斑症状；Ⅱ型在这四种植物上产生系统症状。五个分离物中，ＣＭＶＰ＿２含有五个枝酸组分，其它分离物为四个组分，即ＣＭＶＰ＿２可能含有卫星ＲＮＡ．

omparative studies were made among five CMV isolates cullected from pea(Pisum sativum),hyacinth bean(Dolichus lablab),bean(Phaseolus vulgaris)and red bean(P.angularis),which were namedCMVP_2 CMVP_3,CMVHB,CMVB and CMVRB,respectively.No difference was found in stability insap,aphid transmission,molecular weight of coat protein and electrophoretic mobilities of virions,ex-cept for symptoms in some plant species and RNA components of the virus,In agar gel immunodiffusion tests,the five isolates could produce clear precipitant bands with CMV antiserum and the bandswere confluent.According to the symptoms in Pisum sativum,Vicia faba,Vigna anguiculata and Phaseolusvulgaris,the five isolates could be divided into two groups:group Ⅰwas represented by CMVHB,CMVB and CMVRB,which caused local lesions on inoculated leaves;groupⅡwas reprecented by CMVP_2 and CMVP_3,which produced systemic symptoms.CMUP_2 has five RNA components,whereasother isolates possess four components,so satellite RNAs are presented in CMVP_2.