蚕豆萎蔫病毒──新疆番茄分离株的鉴定

Study on Broad Bean Wilt Virus Isolated from Tomato

在新疆番茄斑驳病株上分离出一种球状病毒，回接到番茄上产生斑驳症状。病毒粒体为２０面体，平均直径２５ｎｍ。经汁液摩擦接种可感染昆诺阿藜、苋色藜、蚕豆、番茄等１８种植物，不感染菜豆、豇豆、豌豆、六叶茄、黄瓜等。可由桃蚜传毒。在琼脂双扩散试验中，能与蚕豆萎蔫病毒（ＢＢＷＶ）抗血清产生明显的沉淀线，与豇豆花叶病毒（ＣｐＭＶ）、黄瓜花叶病毒（ＣＭＶ）抗血清均不发生反应。纯化病毒紫外扫描呈典型的核蛋白吸收叫线，病毒衣壳蛋白由两种蛋白亚基构成，其分子量分别为４５９００和２０４００道尔顿，由１８种氨基酸约２５５个氨基酸残基组成。病毒核酸是双组份的，它们的分子量为１３２００００和２３４００００道尔顿。根据上述结果认为该病毒是蚕豆萎蔫病毒侵染番茄的一个株系。

he isolate 87-35 was iselated from tomato in Xinjiang.After inoculating to tomato inducing mot-tle,the virus particles were isometric,and about 25nm in diameters,The virus infected 18 speciesplant-Chenopodium quinoa,C.amaranticolor,broad bean and tomato etc.It was transmitted by aphid(Myzus percicae)in nonpersistent manner.In the agar double-diffusion tests between the virus andbroad bean wilt virus antiserum,a clear band of precipitate was obseved,but it was not seen reactionbetween the virus and antisera of cowpea mosaic virus,and cucumber mosaic virus,Absorption spectrum of the virus was maximum in 258.5nm and minimum in 241.7nm.The virus particle containedtwo protein subunits,their electrophoresis molecular weights were about 45900 Dalton and 20400 Dal-ton reapectively,And they were composed of l8 kinds amino acids with 255 reeidues,Genome nucleicacid of the virus consisted of two components RNA,their molecular weights were about l320000 Dal-ton and 2340000 Daltun respectively.Accurding to the above results,the isolate 87-35 was identifiedas a strain of Broad Bean Wilt Virus(BBWV).