白血病细胞诱导分化前后对正常人单个核细胞产生TNF_α的影响

The effect of leukemic cells on tumor necrosis factor_α secretion of normal peripheral blood mononuclear cells before and after induction of differentiaion

本文应用拜醇酯、阿糖胞苷、高三尖杉酯碱、维甲酸和维胺酸等５种诱导分化剂诱导８例急性非淋巴细胞白血病患者原代白血病细胞和ＨＬ－６０细胞后，观察细胞（诱导分化前或后）条件培养液对正常人单个核细胞分泌肿瘤坏死因子α（ＴＮＦα）的影响。结果表明，诱导分化前白血病细胞条件培养液可抑制ＴＮＦα分泌，在诱导分化前有抑制作用的６例３０个白血病细胞标本中１７个标本（５６．７％）和ＨＬ－６０细胞在分化成熟后抑制作用丧失，白血病细胞对ＴＮＦα分泌抑制作用丧失与细胞分化有关。

The effect of cell condition medium from 8 patients with acute nonlym- phoid leukemia and from HL-60 on secretion of tumor necrosis factor α( TNFα)of normalperipheral blood mononuclear cells before and after induction of differentiation wasstudied. Inducer included 12-o-tetradecanoyl-phorbol-13-acetate, cytoside arabinoside,homoharringtonine, retinoic acid, retiamide.Cell condition medium from leukemic cellscould suppressed secretion of TNFαbefore induction of differentiation. Loss of suppressionwas found after differentiation in HL-60 and in 17 of 30(56.7%)samples from 6 pa- tients. It was suggested that the loss of suppression of TNFα production by leukemiccells after differentiation was relatted to cell differentiation.