雌二醇、睾酮对健康男性外周血单核细胞HLA－DR、HLA－DQ抗原表达的影响

Effect of 17-β-estradiol and testosterone on the expression of HLA-DR and HLA-DQ antigens of human peripheral blood monocytes

将分离的人单核细胞（Ｍ）在体外分别与雌二醇（Ｅ＿２）、睾酮（Ｔｅ）以及Ｅ＿２＋Ｔｅ共同温育２４小时后，观察Ｍ表面ＨＬＡ－ＤＲ、ＨＬＡ－ＤＱ抗原的表达。结果表明，Ｅ＿２（７．３４×１０￣（－１２）ｍｏｌ／Ｌ）能显著抑制Ｍ表面ＨＬＡ－ＤＲ、ＤＱ抗原的表达，Ｔｅ（３．４７×１０￣（－１１）ｍｏｌ／Ｌ）则能促进Ｍ表面ＨＬＡ－ＤＲ、ＤＱ抗原的表达。Ｅ＿２、Ｔｅ一起作用（浓度分别同上）于Ｍ时，Ｍ表面ＨＬＡ－ＤＲ、ＤＱ抗原表达无明显变化。

Human monocytes(M)of high purity were incubated with estradiol(E_2),testosterone (Te), and E_2+Te,respectively, at 37℃in vitro for 24 hours. The results showedthat E_2 in a concentration of 7.34×10￣(-12)mol/L could inhibit markedly the expressionof HLA-DR and HLA-DQ antigens of the monocytes while Te in a concentration of 3 .47×10￣(-11)mol/L couldpromote the expression. Te + E_2 in concentrations of3.47×10￣(-11)mol/Land 7.3410￣(-12)mol/L,respectively,were found to exert no significant effect on theexpression of HLA-DR and HLA-DQ antigens of the monocytes.