电针家兔“百会穴”对内毒素发热效应及脑脊液、血浆中神经降压素、生长抑素、P物质含量的影响

Observation on ET induced fever response and changes of neurotensin, somatostatin and substance P contents in c.s.f and plasma in rabbits during electric acupuncture on “Bai Hui” point in febrile rabbtis

用精制大肠杆菌内毒素（ｅｎｄｏｔｏｘｉｎ，ＥＴ）给家兔静脉注射，复制发热模型。通过放射性免疫测定方法（ＲＩＡ）观察了发热及电针退热家兔脑脊液中神经降压素（ｎｕｒｏｔｅｎｓｉｎ，ＮＴ），生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ，ＳＳ），Ｐ物质（ｓｕｂｓｔａｎｃｅＰ，ＳＰ）和血浆中ＮＴ含量的变化。结果表明；（１）电针家兔“百会穴”对ＥＴ所致发热有明显的抑制作用。（２）发热和电针抑制发热时家兔脑脊液（ｃ．ｓ．ｆ．）和血浆中ＮＴ、ＳＳ、ＳＰ含量也有明显变化。发热组动物ｃ．ｓ．ｆ．中ＮＴ、ＳＳ含量明显高于对照组（Ｐ＜０．０５）。电针组动物ｃ．ｓ．ｆ．中ＮＴ、ＳＰ含量不仅高于对照组，而且高于发热组（Ｐ＜０．０５）。ＳＳ含量低于对照组和发热组（Ｐ＜０．０１，Ｐ＜０．０５）。血浆ＮＴ含量，发热组低于对照组（Ｐ＜０．０５），电针组高于对照组（Ｐ＜０．０１）。作者认为，家兔ｃ．ｓ．ｆ．中ＮＴ、ＳＳ和ＳＰ及血浆中ＮＴ含量变化与ＥＴ的致热作用有关。电针退热时家兔ｃ．ｓ．ｆ．中ＮＴ、ＳＳ、ＳＰ含量变化在电针退热机制中可能起一定作用。

The fever in rabbits was induced by venous injection of refined of Coliendotoxin(ET). The electric acupuncture was done on “Bai Hui” point of febrile rabbits。The antipyretis effect of electric acupunture was observed and the contents of neurotensin(NT),Somatostatin(ss)and substance P(SP)in both cerebral spinal fluid(c. s. f )andplasma were messeared by radioimmunoassay(RIA)during fever and antipyretis electricacupuncture. The results showed that(1) The electric acupuncture on “Bai Hui” point offebrile rabbits obviously inhibited the fever induced by ET,(2)The contens of NT,SSand SP in both c. s.f and plasma of rabbits can be strongly effected by ET fever andelectric acupuncture. NT content in c. s. f was significantly incresed in both electricacupuncture group and fever group(P<0.01, P<0.05)as compared with control group. The NT content was raised in plasma of electric acupuncture group(P<0.01)and reducedin plasma of fever group(P<0.01 ) as compared with control group. There were muchlower SS Ievels and much higher SP Ievels in c. s. f of electric acupuncture group thanthat of the control group( P<0.01). These results indicated that changes of NT, SSand SP contents in c. s. f was related to the thermoregulatory process during ET feverand antipyretis electric acupuncture.