摘要
本实验对正常及SRS-淋巴瘤小鼠淋巴细胞染色质进行了化学组分、DNaseⅠ有限度消化、染色质附着蛋白释放,热变性的对比分析。结果表明:(1)SRS-淋巴瘤细胞染色质的非组蛋白(non-histonechromosomalproteins,NHCP)和RNA高于正常细胞,提示随着肿瘤的发生染色质转录活性和与转录相关的NHCP呈升高趋势;(2)SRS-淋巴瘤细胞染色质的DNaseⅠ消化率明显高于正常淋巴细胞,提示肿瘤细胞含有较多优先被核酸酶消化的活性染色质;(3)SRS-淋巴瘤细胞较正常淋巴细胞蛋白释放量明显升高,提示参与活性染色质结合的NHCP与染色质活性位点及转录活性有密切相关;(4)SRS-淋巴瘤细胞染色质比正常淋巴细胞增色效应明显,提示肿瘤细胞比正常细胞染色质结构疏松。
Chromatin was extracted from SRS-lymphoma and normal mouselymphocyte by Chiu′s method. Results obtained 1.Their chromatin nuclear acid had notmuch difference, but the values of NHCP/HP and RNA/DNA in mouse SRS-lymphomacells were higher than those in normal lymphocyte.These results showed that the NHCPincrcased significantly with tumor development;2.The ratio of DNase I limited digestionof chromatin in SRS-lymphoma mouse cells were higher than that in normal mouselymphocyte. The results showed that chromatin of SRS-lymphoma mouse cells containedmore DNase I hypersensitive sites than normal mouse lymphocyte;3.The amount ofprotein released clromatin from SRS-lympnolna mouse cells were higher than that fromnormal mouse lymphocyte during DNase I digestion. The results showed that the NHCPspecifically binded active chromatin was closely related to the increasing of active sitesand transcriptional activity of chromatin;4.The thermal denaturation curve showed that thehyperchromic effect of SRS-lylnphoma chromatin was higher than that of normal lymphocyte,the result suggested that the chromatin structure was much loose in SRS-lymphoma cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1994年第4期378-382,共5页
Chinese Journal of Pathophysiology
关键词
淋巴瘤细胞
活性染色质
热变性
Non-histone chromosomal proteins(NHCP)
Active chromatin
DNase Ilimited digestion
Thermal denature