大鼠睾丸与腹腔Mφ的IL－1样物质活性及输精管结扎对其影响

Detection of activity of an interleukin-1-like factor of testicular tissue and abdominal cavity macrophage and influence of vasectomy toward these activities

雄性Ｗｉｓｔａｒ大鼠２１只，分为输精管结扎组（ＶＧ）和假手术组（ＳＯＧ）。术后４个月，以胸腺细胞增殖法检测腹腔Ｍφ和睾丸组织匀浆上清中ＩＬ－１样物质的活性。结果表明，（１）ＩＬ－１物质活性，随睾丸匀浆上清稀释倍数增加而增高，而不同稀释倍数的腹腔Ｍφ培养上清的各测定值均于同一水平。（２）输精管结扎对腹腔Ｍφ培养上清的ＩＬ－１样物质活性无影响，但明显地提高了睾丸匀浆上清的ＩＬ－１样物质活性。本实验结果提示，睾丸组织中存在有ＩＬ－１样物质的抑制因子。

Wistar male rats were divided into vasectomy group (VG )and shamoperation group(SOG ).The activity of interleukin-1-like factor in macrophage andtesticular tissue homogenate super atant were evaluated by murine thymocyte proliferationmethod. The results indieated that:1)Under different supernatant concentration theIL-1-like factor activity from abdominal cavity macrophage were not much differencebetween VG and SOG group.(2)The activity of testicular IL-1-like factor was increasedwith increasing concentration of supernatant.At different supernatant concentration,the average value of IL-1-like factor activity was high in VG than that of SOG.Especiallyat 1 : 32 concentration,there was marked difference between VG and SOG (P<0.05). These results showed that testicular IL-1-like factor activity was markedly increasedafter vasectomy which suggested that there existed an inhibitor of testicular IL-1-likefactor in testis.