结缕草种子打破休眠过程中的代谢调控

Metabolic Control of Zoysiagrass Seed When Breaking Dormancy.

作者测定了结缕草休眠与打破休眠种子发芽过程中磷酸已糖异构酶（ＰＧＩ）、６—磷酸葡萄糖脱氢酶和６—磷酸葡萄糖酸脱氢酶（Ｇ—６—ＰＤＨ和６—ＰＧＤＨ）的联合活性以及苹果酸脱氢酶（ＭＤＨ）的活性。结果表明，打破休眠种子的酶活化较休眠种子快，并且打破休眠种子的Ｇ—６—ＰＤＨ和６—ＰＧＤＨ联合活性及ＭＤＨ活性在发芽过程中显著高于休眠种子。由此证明休眠种子在发芽过程中，其磷酸戊糖途径（ＰＰ）及三羟酸循环（ＴＣＡ）途径没有充分活化。这是结缕草休眠种子不能发芽的原因之一。

Zoysiagrass(Zoysia japonica )has a fine texture and exceptional cold and drought toler- ance. There are a lot of problems in establishing turf by Zoysia seed because of its strong dormancy.In this study, the metabolic control in Zoysiagrass seed during germi- nation is discussed.By analysing the activitied of phosphoglucose isomerase(PGI ),combined glucose 6 phosphate dehydrogenase and 6 phosphogluconate dehydroge- nase(G 6 PDH + 6 PGDH),and malic dehydrogenase(MDH),we found that,activities of G 6 PDH + 6 PGDH and MDH in dormant seed are significantly low- er than these in non dormant seed.This reflected the fact that PP pathway and TCA were not actived enough in dormant Zoysiagrass seed during germination. This is one reason for dormant seed that cant germinate when given suitable conditions.