针刺对拟AD大鼠脑内自由基氧化损伤的影响

The Influence of Acupuncture on Intracerebral Free Radical Oxidation Injury in Simulate AD Rats

目的 运用自由基学说 ,探讨针刺对拟Alzheimer’sDisease(AD)大鼠脑内自由基氧化损伤的影响。方法 应用迷宫观测针刺对拟AD大鼠学习记忆能力的影响 ,应用分光光度法测定大鼠针刺前后大脑皮质中丙二醛 (MDA)含量及谷胱甘肽过氧化物酶 (GSH Px)、超氧化物歧化酶 (SOD)活性的变化。结果 迷宫学习记忆能力测试表明 ,针刺组大鼠成绩好于模型组 (P<0 .0 1) ,且针刺组也优于脑复康组 (P <0 .0 5 )。针刺组大鼠大脑皮质中MDA含量比模型组显著降低 (P <0 .0 1) ,而SOD和GSH Pox活性明显升高 (P <0 .0 1) ,且针刺组与脑复康组之间比较有显著性差异 (P <0 .0 5 )。结论 针刺具有对抗自由基所致的氧化防御系统受损和脂质过氧化水平升高的作用。提示针刺可提高脑内抗氧化能力 ,减轻脑内自由基对神经元的损伤 ,改善AD的病理反应。

Objective To investigate on free radical theory the influence of acupuncture on intracerebral injury due to free radical oxidation in simulate Alzheimer’s Disease(AD) rats. Methods A maze was used to observe the influence of acupuncture on learning and memory ability in simulate AD rats. Malonic dialdehyde(MDA) content, glutathion-peroxidase(GSH-Px) activity and superoxide dismutase(SOD) activity in rat cerebral cortex were determined by spectrophotometry before and after acupuncture. Results A test in maze learning and memory ability showed that it was significantly better in the acupuncture group of rats than in the model group(P<0.01) and also in the Piracetam group(P<0.05). Corticocerebral MDA content was significantly lower(P<0.01) and SOD and GSH-Px activities were significant higher(P<0.01) in the acupuncture group than in the model group. Three were significant differences between the acupuncture and Piracetam groups(P<0.05). Conclusion Acupuncture has a reducing effect on injury to the antioxidation system by free radicals and high lipid peroxidation, suggesting that acupuncture can improve intracerebral antioxidation ability, lessen intracerebral injury to neurons by free radicals and reduce AD lesion.