组织芯片研制的几点体会

A study on manufacture of tissue microarray

目的:进行组织芯片制作可行性探讨,为快速、规范、简便、经济地进行各项科研工作提供一种新的技术方法。方法:制作出组织芯片蜡块,进行HE、免疫组织化学和荧光原位杂交(FISH)染色,对组织芯片免疫组化与普通免疫组化的结果进行比较。结果:各种染色切片上的组织芯基本符合观察需要,芯片免疫组化结果与普通免疫组化结果在统计学上无显著性差异。结论:组织芯片的研制具有高通量、经济节约、减少实验误差的特点,是科研工作中的一种新方法。

Objective: To detect the feasibility of tissue microarray so that a new method could be provided for research. Methods: Two tissue microarray blocks were manufactured and stained on the sections cutting from them by HE,immunohistochemical and fluorescence in situ hybridization(FISH) methods were manufactured and compared with the normal sections. Results: The cores on slides were basically accordant with requirement of research, and there were no significant differences between the results of tissue microarray and normal sections that were dealed with immunohistochemical method. Conclusion: Tissue microarray has characters of high-throughput, economy and low rate of experimental error; it might be a new technique in scientific researches in the future.