金雀异黄素对人乳癌细胞MCF-7增殖和凋亡的影响

The inhibiting effect of genistein on the growth of human breast cancer cells in vitro

目的:采用离体细胞培养的方法,研究大豆异黄酮的主要成分金雀异黄素(GEN)对人乳腺癌MCF-7细胞的增殖抑制作用及其作用机理。方法:MCF-7细胞接受不同浓度的GEN处理,MTT比色法测定GEN抑制MCF-7细胞的量效关系;细胞分裂指数试验用于评价GEN对该细胞的恶性增殖的抑制作用;细胞形态学观察及原位细胞凋亡检测法(TUNEL)用于检测细胞凋亡。结果:GEN的IC30与IC50分别为17.5μmol/L及32.0μmol/L,GEN对MCF-7具有明显的抑制作用,且该抑制作用呈剂量反应关系;各剂量GEN均可抑制MCF-7细胞分裂并且呈剂效关系;细胞形态学观察及TUNEL检测发现GEN处理48h及96h后有明显的凋亡细胞出现,并呈现剂效关系,而本次实验未发现其时效关系。结论:GEN可抑制离体培养MCF-7细胞的增殖,其作用机理可能与GEN诱导细胞凋亡的途径有关。

Objective: To study the inhibiting effect of genistein on the growth of human breast cancer cell lines in vitro and its mechanisms. Methods: Human breast cancer cell lines were cultured in vitro.The proliferation of cells was measured with MTT method and mitotic index curve. Apoptosis was shown with Giemsa and TUNEL methods. Results: Genistein inhibited proliferation of MCF-7 cell lines and the inhibition was dependent on dose within some concentration range and the time range, IC30 and IC50 being 17.5μmol/L and 32.0μmol/L respectively. The inhibition could be shown in the tests of the mitotic index and MTT. GEN could induce apoptosis and the inducement was dependent on dose but not on time, as well. Conclusion: Genistein obviously inhibits proliferation of MCF-7 in vitro and is dependent on dose and time. Genistein's antiproliferous effect on MCF-7 can be realized through inducing apoptosis.